Quick Knockin models are developed by inserting the gene-of-interest in a permissive locus also known as the neutral, or safe zone including Rosa26 and housekeeping gene Hprt. In addition to the quick model generation time, targeting these well-characterized loci gives complete control over the number of copies of the transgene insertion and prevents off-target effects, transgene silencing, or deregulation of neighboring genes.
Quick knockin models can be made to be constitutive or conditionally express the transgene. The expression of the transgene can be tissue-specific or universal. These models are useful for studying the functions of genes, expressing human genes in mice, or validating the specificity of a drug by expressing the protein with mutation or truncation.