CRISPR-Cas9 is a highly versatile system and its utility goes well beyond genome editing. Cas9 Endonuclease Dead or dCas9 whose endonuclease activity is removed can be fused with transcriptional effectors to regulate the gene expression. Similar to Cas9, sgRNA can be used to guide dCas9 to specific genomic locations. dCas9 fused to transcription repressor KRAB (dCas-KRAB) is an effective gene repressor, and dCas9 fused to VP16 a transcriptional activator promotes gene expression.

At TME, we offer a high level of customization to meet your specific needs. Our gene regulatory models are designed to be activated by tetracycline treatment or by light. The activation/regression of the gene of interest can be in all the tissues (universal) or only in the tissue of interest.