Cell-based assays are vital tools for biomedical research. They are designed to understand the complex interplay of signaling pathways and responses within the cellular processes in both normal and disease conditions. As a cost-effective tool, both standardized and custom-made cell-based assays are widely used in numerous applications. For example, during preclinical drug development, cell-based assays enhance compound efficacy screening time and improve productivity. They measure the functional behavior of the cell of interest in response to the candidate compound and evaluate the desired effect (increase cell death, inhibit cell proliferation, inhibit migration). These assays can be customized to best mimic the disease state leading to improved translational success.