Conditional Knockout Models

This strategy allows generating mice with tissue and/or stage-specific deletion of a gene of interest. Conditional models provide precise control of gene expression to yield greater experimental capabilities. Compared to constitutively expressed gene mutations, conditional models provide greater temporal and regional control of gene expression. This strategy is crucial to study genes that are essential for development and their loss can cause embryonic lethality. At TME, we use the Cre-Lox recombinase system to create conditional knockout mice. The floxed mouse with loxP site inserted upstream and downstream of the region of interest is produced using quick knockin technology. These floxed mice are further mated with tissue-specific Cre-expressing mice to produce conditional knockout mice. These models are useful to study the role of genes in a particular organ or tissue. To mimic pathologies caused by gene inactivation in a given cell type. They are also useful to create tissue-specific phenotypes for ubiquitous proteins.

Talk to our experts for your custom model