Knockout models are defined by constitutive inactivation of the gene of interest. Our models are generated by using genome editing tools like CRISPR. Two gRNAs targeting upstream or downstream of the target region/exon(s) of the mouse genome are co-injected with Cas9 into fertilized mouse eggs and knockout pups with critical region deleted are generated. Knockout gene models are fundamental to gene function characterization, induce a phenotype, and create a disease model. They are also important to study the specificity and/or off-target activities of drug candidates.