Soft Agar Colony Formation Assay

Anchorage-independent growth is one of the hallmarks of cellular transformation and uncontrolled growth. Normal epithelial cells require a basement membrane for survival signal and undergo apoptosis if detached from the basement membrane. However, cancerous epithelial cells can undergo transformation to mesenchymal cells and evade the detachment-associated apoptosis signals, and have uncontrolled growth independen of basement membrane in suspension. Colony formation or the soft agar assay is designed to screen the potential of the drug for anchorage-independent cell growth floating in soft agar. At TME, we offer high-throughput screening and growth quantification assay that has been validated for long-term cell growth in suspension or anchorage-independent growth conditions. This assay is also used for cell differentiation, cell transformation, and tumorigenesis studies.

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