Cell Cycle Assay

The cell cycle is the process by which the eukaryotic cells divide into two daughter cells. The cell cycle consists of 2 phases, the Interphase consisting of G1 (Gap 1), S (synthesis), and G2 (Gap 2), and the Mitosis Phase or M phase. During the interphase cell accumulates the energy required for duplication followed by chromosome duplication and division of cytoplasm leading to the formation of two daughter cells. Every step of the cell cycle is highly regulated and checkpoints exist to detect potential DNA damage and allow it to be corrected before cell division. If the DNA damage cannot be repaired, the cells can reversibly stop dividing and enter a senescent stage called G0 or can be targeted for apoptosis. Thus, cell cycle arrest is an effective strategy for oncotherapy. Additionally, monitoring the cell cycle is also crucial to monitor DNA damage which could be an unwanted effect of a new drug.

At TME, we offer flow cytometry-based high-sensitive assays using fluorescent dyes with diverse characteristics like propidium iodide, DAPI, and SiR-Hoechst to evaluate the cell cycle. Our scientists will help will you choose the most appropriate dye that meets your experimental needs.

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